A two-color episomal reporter library tested synthetic ZEV motif-containing promoters in Saccharomyces cerevisiae strain CSY1252, which expresses the ZEV artificial transcription factor. The library was sorted into 12 fluorescence bins under 0 µM beta-estradiol and 1 µM beta-estradiol, producing paired uninduced and induced promoter activity estimates for each sequence.
Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.
Organism
Budding yeast
Taxonomy ID
NCBITaxon:4932
Biosample
UNMAPPED:CSY1252_ZEV_ATF
Reference genome
Not reported / not applicable
Design focus
Synthetic / Motif-focused
Region of interest
Not reported / not applicable
Perturbation & assay details
0 µM beta-estradiol (uninduced) versus 1 µM beta-estradiol (induced)
FACS-seq two-color plasmid reporter with GFP driven by the tested promoter and mCherry driven by constitutive PTEF1. The ZEV artificial transcription factor was expressed from PACT1; each condition was sorted into 12 fluorescence bins with condition-specific gating. Full-length 2 × 300 MiSeq reads were linked to 1 × 75 NextSeq activity estimates through the first 35 bases, and promoter activity is the authors' base-10 log10 GFP:mCherry estimate.
Processed data
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.
Column dictionary · 10 definitions
element_id
Stable package identifier for a QC-passing PZEV promoter sequence.
sequence
Full-length 247-base consensus promoter sequence from the MiSeq run; the paper's prose describes the final library as 246 bp, while the public consensus records are 247 bases long.
identifier_35bp
First 35 bases used to join the full-length MiSeq consensus to the NextSeq activity record.
sequence_length_bp
Length of the consensus promoter sequence in bases.
miseq_cluster_id
Original consensus-cluster identifier from the authors' MiSeq processing pipeline.
miseq_total_bin_reads
Sum of filtered full-length MiSeq reads across the 30 condition/bin-count columns; descriptive coverage only.
miseq_nonzero_bin_count
Number of the 30 MiSeq condition/bin-count columns with at least one read.
activity_uninduced_log10_gfp_mcherry
Author-derived promoter activity with 0 µM beta-estradiol, expressed as base-10 log10 GFP:mCherry ratio.
activity_induced_log10_gfp_mcherry
Author-derived promoter activity with 1 µM beta-estradiol, expressed as base-10 log10 GFP:mCherry ratio.
activation_log10_ratio
Derived induced-minus-uninduced activity; equivalent to the base-10 log of the ratio between the induced and uninduced GFP:mCherry activity ratios.
Quality control
Applied the authors' published processing thresholds and activity-range filters to the public GEO supplements. The NextSeq mean table was generated with a minimum of 20 reads in each condition; retained uninduced activities were within -0.8 to 0.7 and retained induced activities were at least 0.6. Full-length MiSeq consensus rows were linked to NextSeq values using the first 35 bases; unmatched rows and ambiguous duplicate 35-base keys were excluded. Rows containing non-ACGT consensus calls or incorrect length were absent from the public filtered input. The resulting table contains 327,083 QC-passing sequences.
Curation notes
The table is built from the public filtered MiSeq consensus table and public NextSeq mean table, following the repository's merge and filter logic. The MiSeq coverage columns are included for traceability and are not the NextSeq read-depth criterion used by the authors. The two activity columns are conditions rather than biological replicates; activation_log10_ratio is a derived difference. The public consensus sequence length is consistently 247 bases despite the 246-bp description in the article text.