Twenty promoter libraries spanning five promoters and four cHS4/U6-oBC reporter architectures were bottlenecked to estimated 3k, 10k or 30k starting clones in K562 and HEK293. The table reports the source per-barcode activity distribution and its interquartile fold range.
Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.
Organism
Human
Taxonomy ID
NCBITaxon:9606
Biosample
Not reported / not applicable
Reference genome
Not reported / not applicable
Design focus
Other
Region of interest
Not reported / not applicable
Perturbation & assay details
Basal / Untreated
Four piggyBac reporter backbones varied cHS4 insulators and the U6-Tornado/oBC cassette. After controlled population bottlenecking, bulk MPRA activity was measured for individual promoter barcodes and summarized by the activity Q1, Q3 and Q3/Q1 ratio.
Processed data
50 rows per page. Click a cell to inspect its full value.
Visible columns (13 of 13)
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.
Column dictionary · 13 definitions
promoter_id
Promoter identifier.
cell_line
Human cell line used for the integration experiment.
technical_replicate
Bulk MPRA library-preparation replicate.
biological_bottleneck_replicate
Biological replicate and estimated starting population bottleneck.
u6_obc
Whether the U6/oBC cassette is present.
chs4
Whether cHS4 insulators are present.
dna_barcode_count
Number of detected DNA barcodes passing the >10 DNA UMI threshold.
activity_q3
75th percentile of per-barcode RNA/DNA activity.
activity_q1
25th percentile of per-barcode RNA/DNA activity.
activity_iqr_q3_over_q1
Q3 divided by Q1, measuring positional activity variability.
reporter_architecture
Short source identifier for the reporter architecture.
qc_pass
TRUE for finite source summaries passing the ingestion QC filter.
source_table
Supplementary source table used to create the row.
Quality control
The paper retained barcodes with more than 10 DNA UMIs for activity calculations. All 288 author summary rows had at least 16 detected barcodes; rows with non-finite Q3/Q1 fold range (zero Q1, yielding infinity) were excluded from the processed table, leaving 278 finite summaries.
Curation notes
HEK293 and K562 are intentionally represented in one table because this experiment compares positional-effect variability across cell lines; biosample_id is therefore null. The full GSE245191 mBC matrix is retained in raw_data/geo.