The shared synthetic 3′UTR reporter library was co-transfected with a mCherry-tagged Drosophila Smaug expression plasmid in HeLa cells. One RNA output sample was barcode-sequenced against the shared two-replicate plasmid input library.
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Organism
Human
Taxonomy ID
NCBITaxon:9606
Biosample
CVCL:0030
Reference genome
Not reported / not applicable
Design focus
Synthetic / Motif-focused
Region of interest
Not reported / not applicable
Perturbation & assay details
Co-transfection with mCherry-Smaug expression plasmid
The episomal CMV/TO-EGFP reporter library was co-electroporated with pcDNA-D40-mCherry-Smaug. Supplementary Data 7 reports one HeLa RNA/cDNA barcode-count sample; reporter RNA/plasmid activity was normalized to the 4×blank control. Smaug was amplified from Drosophila S2 cDNA, but the assayed biosample is human HeLa.
Processed data
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Column dictionary · 19 definitions
construct_id
Canonical synthetic 3′UTR reporter identity from the source library.
construct_class
Curated class of the reporter identity: blank control, synthetic element combination, let-7 variant, natural let-7 target, or let-7 copy-number variant.
element_position_1
Regulatory element at synthetic 3′UTR position 1; blank for special variant/natural-target identities whose positional encoding is not explicit in the identity label.
element_position_2
Regulatory element at synthetic 3′UTR position 2; blank for special variant/natural-target identities whose positional encoding is not explicit in the identity label.
element_position_3
Regulatory element at synthetic 3′UTR position 3; blank for special variant/natural-target identities whose positional encoding is not explicit in the identity label.
element_position_4
Regulatory element at synthetic 3′UTR position 4; blank for special variant/natural-target identities whose positional encoding is not explicit in the identity label.
regulatory_sequence
132-nt synthetic regulatory-element sequence from Supplementary Data 1, with source capitalization retained.
regulatory_sequence_length_nt
Length of the source regulatory-element sequence in nucleotides.
library_barcode_count
Number of distinct source barcodes assigned to this reporter identity.
qc_barcode_count
Number of barcodes retained after the package read-count QC filter.
plasmid_count_rep1_mean
Mean raw plasmid-input read count per QC-passing barcode in plasmid replicate 1.
plasmid_count_rep2_mean
Mean raw plasmid-input read count per QC-passing barcode in plasmid replicate 2.
cell_type
HeLa with mCherry-Smaug co-transfection; source condition label assigned by the package.
rna_count_mean
Mean raw reporter RNA/cDNA read count per QC-passing barcode in the cell-type sample.
rna_activity_log2_median
Median barcode-level log2 reporter RNA/plasmid activity relative to the 4×blank (BBBB) control.
rna_activity_log2_mean
Arithmetic mean of barcode-level log2 reporter RNA/plasmid activity values.
rna_activity_log2_sd
Sample standard deviation of barcode-level log2 reporter RNA/plasmid activity values.
rna_activity_log2_observations
Number of QC-passing barcode observations contributing to the activity summary.
qc_pass
TRUE for reporter identities with at least three barcodes passing the package QC filter.
Quality control
The authors’ >10-read barcode/plasmid rule was applied to the deposited Smaug RNA counts and both plasmid replicates. The package retained reporter identities with at least three passing barcodes; all 642 identities passed. Activity is relative to the median 4×blank control.
Curation notes
Supplementary Data 7 contains 6,489 Smaug-condition barcode observations and does not provide an independent plasmid-input table; the shared Supplementary Data 2 plasmid counts are used as the authors’ library input reference. No total-RNA, polysome, or 40S Smaug readout was deposited.