Experiment / E46KH1QT53' UTR / RNA Stability MPRA (MPRAu)

Conserved 3′-UTR segment reporter-protein FACS enrichment in BEAS-2B

Massively parallel functional annotation of 3′ untranslated regions

The high-fidelity conserved 3′-UTR fast-UTR library was transduced at low multiplicity into BEAS-2B cells, and cells in the top or bottom 15% of GFP/LNGFR reporter-protein ratios were sorted. The table combines the published segment lists enriched in the high- and low-protein gates.

Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.

Perturbation & assay details

Basal / Untreated

Protein production was read from the GFP/LNGFR fluorescence ratio after anti-LNGFR enrichment and FACS sorting. The authors used approximately 5% transduction efficiency to reduce multiple integrations and re-cloned high/low populations for a second round of sorting.

Processed data

50 rows per page. Click a cell to inspect its full value.

Visible columns (17 of 17)
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.

Column dictionary · 17 definitions
segment_id
Identifier for the conserved 3′-UTR segment or control sequence.
protein_enrichment_class
Published sort-gate class: high reporter protein or low reporter protein.
gene
Gene symbol for the segment when present in Supplementary Data 2.
chromosome
Chromosome for the segment when present in Supplementary Data 2.
published_start
Start coordinate as reported in Supplementary Data 2, when available.
published_end
End coordinate as reported in Supplementary Data 2, when available.
strand
Reported genomic strand, when available.
test_sequence_dna_5to3
Published test insert in DNA alphabet and reporter orientation, when available.
sequence_length_nt
Length of the published test insert, when available.
reads_high_gate
Normalized reads assigned to the high reporter-protein sort gate.
reads_low_gate
Normalized reads assigned to the low reporter-protein sort gate.
total_normalized_reads
Sum of high- and low-gate normalized reads used for the paper’s coverage filter.
high_to_low_ratio
High-gate divided by low-gate normalized reads; blank when the denominator is zero.
low_to_high_ratio
Low-gate divided by high-gate normalized reads; blank when the denominator is zero.
enrichment_ratio
Ratio in the direction of the row’s published enrichment class; blank for infinite ratios.
enrichment_ratio_infinite
True when the class-defining denominator is zero in the source data.
reported_enrichment_ratio
Ratio exactly as printed in Supplementary Data 4, including Inf.

Quality control

The authors classified segments at high/low or low/high read ratios of at least 10 and excluded segments with fewer than 500 total normalized reads across the two sort gates. The package retains 873 published enriched rows (568 high and 305 low); every retained row has at least 500 total normalized reads. Seven control-only IDs lack a matching sequence record in Supplementary Data 2 and are retained with blank annotation fields.

Curation notes

The source workbook contains only the enriched high/low lists rather than all library segments. The seven CN1_CT control IDs are retained because they pass the paper’s read/ratio criteria, although their sequence and coordinates are not present in Supplementary Data 2.

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