The high-fidelity conserved fast-UTR library tested 160-nt segments from conserved human 3′-UTRs in WiDr-tTA cells. Reporter RNA/cDNA-to-genomic-DNA ratios were summarized as segment-level steady-state mRNA activity.
Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.
Organism
Human
Taxonomy ID
NCBITaxon:9606
Biosample
CVCL:2760
Reference genome
NCBI36/hg18
Design focus
Region-focused
Region of interest
Not reported / not applicable
Perturbation & assay details
Basal / Untreated
The fast-UTR BTV reporter was delivered through lentiviral transduction into tTA-expressing cells. High-fidelity oligonucleotide pools contained 160-nt conserved human 3′-UTR segments and random octamer clone indexes; paired-end Illumina reads from cellular reporter RNA/cDNA and genomic DNA were normalized per sample, and median RNA/DNA ratios were summarized as log2 steady-state mRNA relative to the median segment activity.
Processed data
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Visible columns (9 of 9)
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.
Column dictionary · 9 definitions
segment_id
Identifier for the tested conserved 3′-UTR segment.
gene
Gene symbol associated with the tested 3′-UTR segment.
chromosome
Chromosome from the source supplementary table.
published_start
Start coordinate as reported in Supplementary Data 2.
published_end
End coordinate as reported in Supplementary Data 2.
strand
Reported genomic strand of the source 3′-UTR.
test_sequence_dna_5to3
Published 160-nt reporter test insert in DNA alphabet, in 5′→3′ insert orientation.
sequence_length_nt
Length of the published test insert in nucleotides.
log2_steady_state_mrna
Published log2 steady-state reporter mRNA activity for WiDr, relative to the median of all segments tested in that cell type.
Quality control
The source reports medians from at least 10 clones per segment after the authors’ clone/read filtering. For this cell type, the package retains 2603 rows with a finite published log2 steady-state mRNA value and a matching sequence record; nd values and unjoinable records are excluded.
Curation notes
The tTA-expressing derivative was made by transducing the parental cell line; the exact clone is not specified. Supplementary Data 2 contains 2,835 published sequence records, whereas the article methods describe 2,828 oligos/3,000 conserved elements; sequence and coordinate fields are preserved as reported. Data2 coordinates are from the NCBI36/hg18-era design table. Cellosaurus identifies WiDr as a problematic line with a documented HT-29 derivative history; the paper also states that cell lines were not authenticated or tested for mycoplasma contamination.