The high-fidelity conserved fast-UTR library tested 160-nt segments from conserved human 3′-UTRs in BEAS-2B-tTA cells. Reporter RNA/cDNA-to-genomic-DNA ratios were summarized as segment-level steady-state mRNA activity.
Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.
Organism
Human
Taxonomy ID
NCBITaxon:9606
Biosample
CVCL:0168
Reference genome
NCBI36/hg18
Design focus
Region-focused
Region of interest
Not reported / not applicable
Perturbation & assay details
Basal / Untreated
The fast-UTR BTV reporter was delivered through lentiviral transduction into tTA-expressing cells. High-fidelity oligonucleotide pools contained 160-nt conserved human 3′-UTR segments and random octamer clone indexes; paired-end Illumina reads from cellular reporter RNA/cDNA and genomic DNA were normalized per sample, and median RNA/DNA ratios were summarized as log2 steady-state mRNA relative to the median segment activity.
Processed data
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Visible columns (9 of 9)
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.
Column dictionary · 9 definitions
segment_id
Identifier for the tested conserved 3′-UTR segment.
gene
Gene symbol associated with the tested 3′-UTR segment.
chromosome
Chromosome from the source supplementary table.
published_start
Start coordinate as reported in Supplementary Data 2.
published_end
End coordinate as reported in Supplementary Data 2.
strand
Reported genomic strand of the source 3′-UTR.
test_sequence_dna_5to3
Published 160-nt reporter test insert in DNA alphabet, in 5′→3′ insert orientation.
sequence_length_nt
Length of the published test insert in nucleotides.
log2_steady_state_mrna
Published log2 steady-state reporter mRNA activity for BEAS-2B, relative to the median of all segments tested in that cell type.
Quality control
The source reports medians from at least 10 clones per segment after the authors’ clone/read filtering. For this cell type, the package retains 2574 rows with a finite published log2 steady-state mRNA value and a matching sequence record; nd values and unjoinable records are excluded.
Curation notes
The tTA-expressing derivative was made by transducing the parental cell line; the exact clone is not specified. Supplementary Data 2 contains 2,835 published sequence records, whereas the article methods describe 2,828 oligos/3,000 conserved elements; sequence and coordinate fields are preserved as reported. Data2 coordinates are from the NCBI36/hg18-era design table.