Experiment / E24B4SDGVWhole-Genome STARR-seq (WHG-STARR-seq)

HeLa-S3 whole-genome STARR-seq inhibitor-response differential analysis

Resolving systematic errors in widely-used enhancer activity assays in human cells enables genome-wide functional enhancer characterization

This derived comparison tests differential activity between the inhibitor-treated and untreated HeLa-S3 ORI STARR-seq screens described above. Peak calls overlapping by at least 85% were collapsed and evaluated with the paper's hypergeometric differential test; the table preserves all published comparison intervals and labels the FDR-supported direction.

Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.

Perturbation & assay details

Comparison: 1 µM C16 + 1 µM BX-795 versus no inhibitors

Derived from the two episomal ORI-based genome-wide STARR-seq conditions; it is not an additional transfection. The paper combined/collapsed peaks when they overlapped by at least 85%, used the maximum count in each combined region for the hypergeometric test, and adjusted over- and under-activity p-values to FDRs.

Processed data

50 rows per page. Click a cell to inspect its full value.

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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.

Column dictionary · 17 definitions
element_id
Stable identifier constructed from the unique source chromosome/start/end interval.
chromosome
Human chromosome name from the hg19-aligned comparison interval.
start_hg19
Comparison interval start coordinate as reported by GEO.
end_hg19
Comparison interval end coordinate as reported by GEO.
interval_length_bp
Interval length in base pairs calculated as end - start + 1 from the reported coordinates.
log2fc_inhibitor_vs_no_inhibitor
Published log2 fold-change for inhibitor-treated versus untreated STARR-seq signal.
p_over_fdr
FDR-adjusted p-value for inhibitor-associated over-activity.
p_under_fdr
FDR-adjusted p-value for inhibitor-associated under-activity.
inhibitor_effect_direction
Direction from the sign of the published log2 fold-change: inhibitor_higher, inhibitor_lower, or no_change.
differential_call_fdr_0_05
Conservative direction-specific call using the corresponding over/under FDR < 0.05; otherwise not_significant.
inhibitor_peak_id
Published inhibitor-screen peak ID overlapping the differential interval, when present.
no_inhibitor_peak_id
Published untreated-screen peak ID overlapping the differential interval, when present.
inhibitor_peak_rank
Numeric rank parsed from inhibitor_peak_id, when present.
no_inhibitor_peak_rank
Numeric rank parsed from no_inhibitor_peak_id, when present.
nearest_tss_ensembl75
Ensembl v75 gene identifier for the nearest annotated transcription start site.
nearest_gene
Gene symbol/name associated with the nearest Ensembl v75 transcription start site.
source_table
Filename of the deposited GEO differential table used to create this row.

Quality control

The source comparison was built from the paper's filtered genome-wide peak calls and uses FDR-adjusted over- and under-activity p-values. Package QC additionally required a valid positive-width hg19 interval, finite log2 fold-change, FDR values in [0,1], and unique interval identifiers. All 12,424/12,424 source comparison rows passed and no rows were removed. Non-significant rows are retained so researchers can distinguish absence of differential evidence from missing data.

Curation notes

This child package is explicitly a derived analysis of GSM2683035/36 versus GSM2683039/40, not an independent wet-lab experiment. Empty inhibitor/no-inhibitor peak ID fields indicate that the published differential interval was present in only one condition's peak set. The raw GEO differential table is retained unchanged in raw_data.

Cite OpenMPRA

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Please also cite the source studies when using their data.