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Experiment / E4RFNEE42Integrated lentiMPRA

Integrated 5'/5' WT lentiMPRA of HepG2 candidate liver enhancers

A systematic evaluation of the design and context dependencies of massively parallel reporter assays

The common 2,440-element library was packaged with wild-type lentiviral integrase, placing 171-bp candidate enhancers upstream of a minimal promoter and linked barcodes in the reporter 5' UTR. Three independent HepG2 infections were profiled by matched DNA and RNA barcode sequencing.

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Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.

Organism
Human
Taxonomy ID
NCBITaxon:9606
Biosample
CVCL:0027
Reference genome
hg19
Design focus
Region-focused
Region of interest
Not reported / not applicable
Perturbation & assay details

Basal / Untreated

Wild-type-integrase lentiMPRA with enhancer upstream of the minimal promoter and barcode in the reporter 5' UTR, reducing enhancer-to-barcode distance; random chromosomal integration and matched DNA/RNA barcode sequencing.

Processed data

50 rows per page. Click a cell to inspect its full value.

Visible columns (20 of 20)
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.

Column dictionary · 20 definitions
element_id
Published identifier for the tested 171-bp enhancer or control element.
element_type
Source element class: test, pos control, or neg control.
chromosome
Human hg19 chromosome for the source element; blank for synthetic controls without a genomic coordinate.
start
1-based hg19 start coordinate of the 171-bp source element; blank when unavailable.
end
1-based hg19 end coordinate of the 171-bp source element; blank when unavailable.
sequence
The 171-bp DNA sequence tested in the assay.
sequence_length_bp
Length of sequence in base pairs.
activity_mean_log2_rna_dna
Authors' activity score: mean log2 RNA/DNA after per-replicate median normalization; the source excludes the low-quality replicate 1 for 5'/3' MT and 3'/3' MT.
replicate_1_normalized_rna_cpm
Normalized RNA barcode count for replicate 1, reported as counts per million in the authors' pipeline.
replicate_1_normalized_dna_cpm
Normalized DNA barcode count for replicate 1, reported as counts per million in the authors' pipeline.
replicate_1_log2_rna_dna_ratio
Raw per-replicate log2(RNA/DNA) activity value for replicate 1, before the across-replicate median normalization used for the published mean.
replicate_1_unique_barcodes
Number of unique barcodes assigned to the element in replicate 1.
replicate_2_normalized_rna_cpm
Normalized RNA barcode count for replicate 2, reported as counts per million in the authors' pipeline.
replicate_2_normalized_dna_cpm
Normalized DNA barcode count for replicate 2, reported as counts per million in the authors' pipeline.
replicate_2_log2_rna_dna_ratio
Raw per-replicate log2(RNA/DNA) activity value for replicate 2, before the across-replicate median normalization used for the published mean.
replicate_2_unique_barcodes
Number of unique barcodes assigned to the element in replicate 2.
replicate_3_normalized_rna_cpm
Normalized RNA barcode count for replicate 3, reported as counts per million in the authors' pipeline.
replicate_3_normalized_dna_cpm
Normalized DNA barcode count for replicate 3, reported as counts per million in the authors' pipeline.
replicate_3_log2_rna_dna_ratio
Raw per-replicate log2(RNA/DNA) activity value for replicate 3, before the across-replicate median normalization used for the published mean.
replicate_3_unique_barcodes
Number of unique barcodes assigned to the element in replicate 3.

Quality control

Author QC was retained: barcode-to-element associations required at least three independent observations and at least 90% of barcode sequence mapped to one element; only barcodes observed in both the DNA and RNA sample were counted; UMI-collapsed counts were normalized to counts per million; and replicate RNA/DNA ratios were median-normalized before averaging. Package QC retained only rows with a valid A/C/G/T 171-bp sequence, finite published mean and replicate activity values, and finite source RNA, DNA, and unique-barcode fields for all three replicates. 2429 rows passed from 2429 source mean-score rows; 0 incomplete/missing rows were excluded. No extra minimum-barcode cutoff was imposed because the paper uses its measured element set for assay comparisons; observed barcode counts remain in the table.

Curation notes

All 2429 complete elements in the authors' 2429-row all-assay mean table were retained; the source activity mean is the published median-normalized score.

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