Mouse embryonic stem cells were differentiated into mouse neural precursor cells and profiled with the episomal primeTF reporter library. The table reports reporter activity changes between the mNPC and mESC states for the 100-TF panel.
Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.
Organism
Mouse
Taxonomy ID
NCBITaxon:10090
Biosample
CL:0011020
Reference genome
Not reported / not applicable
Design focus
Synthetic / Motif-focused
Region of interest
Not reported / not applicable
Perturbation & assay details
In vitro differentiation of E14TG2a mESCs into mouse neural precursor cells.
The primeTF reporter plasmid library was assayed in the mESC and differentiated mNPC states. Reporter RNA barcode activity was normalized to plasmid DNA and analyzed with primetime; paired chromVAR and TOBIAS values are included for comparison.
Processed data
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Visible columns (14 of 14)
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.
Column dictionary · 14 definitions
reporter_tf
Name of the synthetic reporter and assayed transcription factor
condition
Condition label from Dataset EV1
control_state
mESC baseline state
tested_state
mESC-derived mNPC state
primetime_log2_fc
PrimeTF reporter activity log2 fold change for mNPC versus mESC
primetime_adjusted_p_value
Adjusted p-value supplied for the primeTF reporter activity comparison
primetime_significance
Source significance call: Upregulated, Downregulated, or NS
chromvar_difference
Difference in chromVAR TF-associated accessibility score for the paired ATAC-seq comparison
chromvar_adjusted_p_value
Adjusted p-value for the paired chromVAR comparison
chromvar_significance
Source chromVAR significance call
tobias_log2_fc
TOBIAS ATAC-seq TF activity log2 fold change
tobias_p_value
TOBIAS comparison p-value
tobias_significance
Source TOBIAS significance call
qc_status
Row-level package QC status; passed means primeTF effect and adjusted p-value were numeric
Quality control
The paper used primetime v1.0 quality checks for low plasmid-DNA bleedthrough, high barcode correlation, and high biological-replicate correlation. Processed rows were retained only when Dataset EV1 supplied numeric primeTF log2 fold-change and adjusted p-value values; all 99 source rows for this condition passed this package's numeric-value filter. NS is a statistical call, not a QC failure.
Curation notes
The paper describes this as a more complex differentiation setting without a single ground-truth TF response. NFIA is specifically noted as a non-prime reporter and a method-specific outlier; no missing reporter values were imputed.