QeFS in mef2:CD2− FCM-depleted embryonic cells

The same pooled genomically integrated enhancer-reporter library was assayed in stage 11–12 embryos crossed to a mef2[IE-D5]:CD2 driver. The CD2-negative, fusion-competent-myoblast-depleted fraction was quantified by targeted reporter RNA and DNA sequencing with UMI-tagged reporter molecules and combined across two collection series.

Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.

Organism: Fruit fly
Taxonomy ID: NCBITaxon:7227
Biosample: UNMAPPED:embryonic_cells_depleted_of_fusion_competent_myoblasts
Reference genome: Not reported / not applicable
Design focus: Synthetic / Motif-focused
Region of interest: Not reported / not applicable

Perturbation & assay details

Wild-type versus systematic single, pairwise, and three-way mutations of Deaf1, Schlank, ZIPIC, and/or CG12236-PB binding-site motifs; Act57B and β3Tub60D positive-control constructs were included.

Quantitative enhancer-FACS-Seq (QeFS) uses site-specific φC31 genomic integration in Drosophila, 8-mer enhancer barcodes, and degenerate 20-mer tags in the reporter 3′ UTR. FACS separates the CD2-marked cell population rather than binning reporter fluorescence; targeted RNA/DNA UMI counts calculate DNA-normalized reporter activity across six mef2:CD2− collection-run labels from two collection series.

Quality control

Applied the authors' stated filters to the GEO fullFiltered per-tag tables: removed individual measurements with fewer than 65 DNA UMIs and retained construct groups with at least 4 qualifying tag-level measurements. The resulting construct-level means, standard deviations, totals, and standard errors were cross-checked against Additional file 4/Table S4; only constructs passing these filters are present.

Curation notes

The table contains 91 QC-passing constructs and is aggregated from the GEO MEFCD2MINUS fullFiltered per-tag measurements, combining two collection series. This is the CD2-negative FCM-depleted proxy used by the authors; it is not the scarce mef2:CD2+ FCM fraction, for which no comparable public processed table was deposited. The sequence is the representative sequence-table record rather than every barcode-linked reporter molecule; all qualifying reporter tags remain available in raw_data. The ChIPCRM5792 series has no QC-passing WT construct, so WT-relative fields are null for that parent.

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QeFS in mef2:CD2− FCM-depleted embryonic cells

Processed data

Quality control

Curation notes

Cite OpenMPRA

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