Experiment / E06BB3E62Standard STARR-seq

HepG2 random-enhancer STARR-seq

Sequence determinants of human gene regulatory elements

A random N170 enhancer library was assayed in HepG2 cells using an episomal STARR-seq reporter. The table contains motif-match RNA/input activity effects for the 1,121 deposited motif models.

Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.

Perturbation & assay details

Basal / Untreated

Episomal pCpG-free-Sasaki-SS STARR-seq library containing synthetic random N170 enhancer sequences in the reporter 3′ UTR. RNA was collected 24 h after transfection; source motif matching used MOODS at p=1e-6 and the activity output is PsiLFC.

Processed data

50 rows per page. Click a cell to inspect its full value.

Visible columns (8 of 8)
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.

Column dictionary · 8 definitions
motif_model
Position-frequency-matrix model name
tf
Transcription-factor label associated with the motif model
total_match_count
HepG2 total motif-match count in RNA
input_match_count
HepG2 input-DNA motif-match count
log2_rna_input_effect
HepG2 PsiLFC/log2 RNA-over-input activity effect
effect_direction
Sign-based direction of the activity effect
motif_match_pvalue_threshold
MOODS motif-match p-value threshold used by the source
source_accession
GEO source accession

Quality control

The GEO file contains 1,121 motif models. Package QC required finite effects, nonnegative integer match counts, and total-plus-input counts of at least 5; all 1,121 rows passed and were retained.

Curation notes

The table is the single-source HepG2 random-enhancer motif-match output; it contains no GP5d comparison columns because the source file provides only this cell-line condition.

Cite OpenMPRA

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Please also cite the source studies when using their data.