A focused STARR-seq library made from 29 BACs covering approximately 5 Mb of Drosophila melanogaster genomic sequence was screened in S2 cells with nine core-promoter reporters: RpS12, DSCP, eEF1delta, x16, NipB, pnr, Hsp70, eve, and eve-long. The table contains the author-called peak summits and input-normalized activities, annotated to the BAC interval from Supplementary Table 16.
Processed tables are specific to each experiment. Column names, units, measurements, and table structure are not standardized across the database. Check this experiment’s column definitions and quality-control notes before comparing or combining data.
Organism
Fruit fly
Taxonomy ID
NCBITaxon:7227
Biosample
CVCL:Z232
Reference genome
dm3
Design focus
Region-focused
Region of interest
Not reported / not applicable
Perturbation & assay details
Basal / Untreated
Episomal plasmid STARR-seq reporters were built from a common BAC-derived genomic-fragment library, with the core-promoter sequence swapped between constructs. The authors balanced the 29 BACs, normalized the BAC representation using the corresponding inputs, and processed the focused screens as STARR-seq peak calls; the compact table retains all nine deposited core-promoter contexts.
Processed data
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Filters apply to this table only. The CSV download contains the complete processed table; filtered rows are available through the API.
Column dictionary · 17 definitions
element_id
Stable identifier composed of cell type, core promoter, chromosome, and reported summit.
core_promoter
Core-promoter sequence carried by the focused STARR-seq reporter.
cell_type
Drosophila cell context; S2 for this experiment.
library_scope
Focused BAC-derived genomic library covering approximately 5 Mb across 29 BACs.
chromosome
dm3 chromosome or heterochromatin contig from the deposited peak call.
summit
Peak summit coordinate as reported in the GEO file.
peak_window_501bp_start
Start of the reported-coordinate +/-250-bp window around the summit.
peak_window_501bp_end
End of the reported-coordinate +/-250-bp window around the summit.
enrichment_over_input
Author-deposited STARR-seq enrichment over input at the peak summit after focused-library normalization.
log2_enrichment_over_input
Derived log2 of enrichment_over_input.
p_value
Author peak-call p-value from the input comparison; zero denotes underflow in the source file.
specificity_class_vs_RpS12_DSCP
Not assessed for the focused BAC comparison.
bac_id
BAC identifier containing the reported summit, resolved from Supplementary Table 16 coordinates.
replicate_status
Single focused BAC screen/file for each core-promoter context; not a replicate mean.
source_accession
GEO series and sample accession provenance for the source peak file.
source_file
Relative path to the compressed source peak file in raw_data.
qc_status
Packaging QC result; all retained rows are pass.
Quality control
The authors restricted analysis to fragments originating from the 29 BACs, adjusted input and STARR-seq data to equalize BAC representation, and then applied the STARR-seq peak-calling pipeline. For packaging, rows were retained only when they had exactly four tab-separated fields, a chromosome-like dm3 coordinate, an integer summit >=1, finite positive enrichment, and a finite p-value in [0,1]; p=0 was retained as numerical underflow. All 2,920 deposited peak rows passed these checks and no rows were rejected; all 2,920 summits mapped to one BAC interval from Supplementary Table 16.
Curation notes
The focused screens are distinct from the genome-wide screens because they use a balanced 29-BAC library and input-based BAC equalization. The paper reports high concordance among the core-promoter comparisons and uses the DSCP, wild-type eve, and eve-long controls to assess functional similarity. Source data include nine BAC peak files (one per promoter context); the GEO sample records do not label biological replicates, so values are reported as single focused-screen peak calls.